Characterization of vesicles secreted from insulinoma NIT-1 cells.

  title={Characterization of vesicles secreted from insulinoma NIT-1 cells.},
  author={Hyo Sun Lee and Jaeho Jeong and Kong-Joo Lee},
  journal={Journal of proteome research},
  volume={8 6},
Insulinoma NIT-1, an insulin-secreting mouse cell line, secretes vesicles in response to glucose or calcium. These vesicles, like exosomes, are relatively homogeneous (30-100 nm). We analyzed their protein profiles employing one-dimensional SDS gel electrophoresis combined with nanoLC-ESI-q-TOF tandem mass spectrometry, and searched for post-translational modifications (PTMs) using MOD(i) algorithm. We identified 270 proteins which matched at least two peptides reproducibly in duplicate runs… CONTINUE READING


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