Characterization of vaginal Lactobacillus species by rplK -based multiplex qPCR in Russian women.

@article{Demkin2017CharacterizationOV,
  title={Characterization of vaginal Lactobacillus species by rplK -based multiplex qPCR in Russian women.},
  author={Vladimir V. Demkin and Stanislav I Koshechkin},
  journal={Anaerobe},
  year={2017},
  volume={47},
  pages={
          1-7
        }
}
We describe a multiplex qPCR assay for identification and quantitative assessment of a set of vaginal Lactobacillus species, including L. acidophilus, L. crispatus, L. gasseri, L. helveticus, L. iners, and L. jensenii. The assay extends the previously developed qPCR method for Lactobacillus detection and total quantification based on targeting the rplK gene. Both assays use only single pair of primers and a set of probes combined in three reactions, comprising a vaginal Lactobacillus diagnostic… CONTINUE READING
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