Characterization of vaccinia virus intracellular cores: implications for viral uncoating and core structure.

@article{Pedersen2000CharacterizationOV,
  title={Characterization of vaccinia virus intracellular cores: implications for viral uncoating and core structure.},
  author={Kia Pedersen and Eric J. Snijder and Sibylle Schleich and Norbert Roos and Gareth Griffiths and Jacomine Krijnse Locker},
  journal={Journal of virology},
  year={2000},
  volume={74 8},
  pages={3525-36}
}
The entry of vaccinia virus (VV) into the host cell results in the delivery of the double-stranded DNA genome-containing core into the cytoplasm. The core is disassembled, releasing the viral DNA in order to initiate VV cytoplasmic transcription and DNA replication. Core disassembly can be prevented using the VV early transcription inhibitor actinomycin D (actD), since early VV protein synthesis is required for core uncoating. In this study, VV intracellular cores were accumulated in the… CONTINUE READING