Characterization of universal small-subunit rRNA hybridization probes for quantitative molecular microbial ecology studies.

@article{Zheng1996CharacterizationOU,
  title={Characterization of universal small-subunit rRNA hybridization probes for quantitative molecular microbial ecology studies.},
  author={Dasheng Zheng and Elizabeth W Alm and David A. Stahl and Lutgarde M. Raskin},
  journal={Applied and environmental microbiology},
  year={1996},
  volume={62 12},
  pages={4504-13}
}
Universal oligonucleotide hybridization probes targeting the small-subunit rRNA are commonly used to quantify total microbial representation in environmental samples. Universal probes also serve to normalize results obtained with probes targeting specific phylogenetic groups of microorganisms. In this study, six universal probes were evaluated for stability of probe-target duplexes by using rRNA from nine organisms representing the three domains of Bacteria, Archaea, and Eucarya. Domain… CONTINUE READING
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