Characterization of tynorphin, a potent endogenous inhibitor of dipeptidyl peptidaseIII

@article{Yamamoto2000CharacterizationOT,
  title={Characterization of tynorphin, a potent endogenous inhibitor of dipeptidyl peptidaseIII},
  author={Yukio Yamamoto and Junpei Hashimoto and Mariko Shimamura and Teruhide Yamaguchi and Tadahiko Hazato},
  journal={Peptides},
  year={2000},
  volume={21},
  pages={503-508}
}
To find a more effective inhibitor than spinorphin (LVVYPWT), an endogenous factor derived from bovine spinal cord, we synthesized spinorphin analogues and assayed their inhibitory activity toward DPPIII among enkephalin-degrading enzymes. Tynorphin (VVYPW), an N-terminal and C-terminal truncated form of spinorphin, exhibited more potent inhibitory activity and an IC50 value of 0.086 +/- 0.05 microg/ml (n = 4), whereas structures smaller than four amino acid residues exhibited almost no or less… 
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