Characterization of two-photon excitation fluorescence lifetime imaging microscopy for protein localization.

@article{Chen2004CharacterizationOT,
  title={Characterization of two-photon excitation fluorescence lifetime imaging microscopy for protein localization.},
  author={Ye Chen and Ammasi Periasamy},
  journal={Microscopy research and technique},
  year={2004},
  volume={63 1},
  pages={72-80}
}
Two-photon excitation fluorescence resonance energy transfer (2P-FRET) imaging microscopy can provide details of specific protein molecule interactions inside living cells. Fluorophore molecules used for 2P-FRET imaging have characteristic absorption and emission spectra that introduce spectral cross-talk (bleed-through) in the FRET signal that should be removed in the 2P-FRET images, to establish that FRET has actually occurred and to have a basis for distance estimations. These contaminations… CONTINUE READING

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