Characterization of two acidic β-glucosidases and ethanol fermentation in the brown rot fungus Fomitopsis palustris.

@article{Okamoto2011CharacterizationOT,
  title={Characterization of two acidic β-glucosidases and ethanol fermentation in the brown rot fungus Fomitopsis palustris.},
  author={Kenji Okamoto and Yuko Sugita and Natsumi Nishikori and Yasuyuki Nitta and Hideshi Yanase},
  journal={Enzyme and microbial technology},
  year={2011},
  volume={48 4-5},
  pages={359-64}
}
Two acidic β-glucosidases (βGI and βGII) from the brown rot fungus Fomitopsis palustris were purified to homogeneity by several chromatographic steps. βGI and βGII had molecular weights of 130 and 213 kDa, respectively, and exhibited optimum activity at pH 2.5 and 55°C. The K(m) values of βGI and βGII for p-nitrophenyl-β-d-glucopyranoside were 0.706 and 0.971 mM, respectively. Although the effect of metal ions and inhibitors differed between the two enzymes, both β-glucosidases exhibited… CONTINUE READING