Characterization of triacylglycerol biosynthesis in subcellular fractions of an oleaginous fungus, Mortierella ramanniana var. angulispora.

@article{Pillai1998CharacterizationOT,
  title={Characterization of triacylglycerol biosynthesis in subcellular fractions of an oleaginous fungus, Mortierella ramanniana var. angulispora.},
  author={M Ramasamy Pillai and Milan {\vC}ert{\'i}k and Taketoshi Nakahara and Yasushi Kamisaka},
  journal={Biochimica et biophysica acta},
  year={1998},
  volume={1393 1},
  pages={
          128-36
        }
}
Triacylglycerol (TG) biosynthetic enzymes were characterized in subcellular fractions of an oleaginous fungus, Mortierella ramanniana var. angulispora. When the membrane or lipid body fraction of this fungus was incubated with [14C]oleoyl-CoA without adding exogenous acyl acceptors, radioactivity was incorporated predominantly into TG, indicating that diacylglycerol acyltransferase (DGAT) used endogenous diacylglycerol to incorporate [14C]oleoyl-CoA into TG. Adding glycerol 3-phosphate or… CONTINUE READING

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