Characterization of transformation-deficient mutants of Acinetobacter calcoaceticus.

  title={Characterization of transformation-deficient mutants of Acinetobacter calcoaceticus.},
  author={Ronald Palmen and Ben Vosman and Ruben G Kok and Julie van der Zee and Klaas J. Hellingwerf},
  journal={Molecular microbiology},
  volume={6 13},
Three Acinetobacter calcoaceticus transformation-deficient mutants, obtained by insertional mutagenesis with the nptll gene, have been characterized physiologically. One mutant (AAC211) was found to be completely transformation deficient, while two others, AAC213 and AAC214, were severely impaired in transformation efficiency (100-1000 times lower than the wild type). The latter applied to both chromosomal as well as plasmid DNA. Analysis of the chromosomal DNA fragments flanking the nptll gene… CONTINUE READING

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