Characterization of toad liver glutathione transferase.


The major form of glutathione transferase from the toad liver previously designed as Bufo bufo liver GST-7.6 (A. Aceto, B. Dragani, T. Bucciarelli, P. Sacchetta, F. Martini, S. Angelucci, F. Amicarelli, M. Miranda and C. Di Ilio, Biochem. J. 289 (1993) 417-422) has been characterized. According to its partial amino acid sequence, the toad enzyme may be included in the pi class GST and named bbGST P2-2. However, bbGST P2-2 appears to be immunologically, structurally and kinetically distinct from any other members of pi family, including bbGST P1-1, suggesting that it may constitute a subset of pi class GST. The data support the hypothesis that the transition from aquatic to terrestrial life causes a switch of the GST amphibian pattern promoting the expression of a GST form (bbGST P2-2) able to counteract, with higher efficiency, the toxic effects of reactive metabolites of oxidative metabolism and those of hydrophobic xenobiotics.

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@article{Bucciarelli1999CharacterizationOT, title={Characterization of toad liver glutathione transferase.}, author={T. Bucciarelli and Paolo Sacchetta and Alfonso Pennelli and Lisa Cornelio and Romeo Romagnoli and Sonia Melino and Raffaele Petruzzelli and Carmine di Ilio}, journal={Biochimica et biophysica acta}, year={1999}, volume={1431 1}, pages={189-98} }