Characterization of the yeast DGK1-encoded CTP-dependent diacylglycerol kinase.

  title={Characterization of the yeast DGK1-encoded CTP-dependent diacylglycerol kinase.},
  author={Gil-Soo Han and Laura O'Hara and Symeon Siniossoglou and George M Carman},
  journal={The Journal of biological chemistry},
  volume={283 29},
The Saccharomyces cerevisiae DGK1 gene encodes a diacylglycerol kinase enzyme that catalyzes the formation of phosphatidate from diacylglycerol. Unlike the diacylglycerol kinases from bacteria, plants, and animals, the yeast enzyme utilizes CTP, instead of ATP, as the phosphate donor in the reaction. Dgk1p contains a CTP transferase domain that is present in the SEC59-encoded dolichol kinase and CDS1-encoded CDP-diacylglycerol synthase enzymes. Deletion analysis showed that the CTP transferase… CONTINUE READING