Characterization of the total and viable bacterial and fungal communities associated with the International Space Station surfaces

@article{ChecinskaSielaff2019CharacterizationOT,
  title={Characterization of the total and viable bacterial and fungal communities associated with the International Space Station surfaces},
  author={Aleksandra Checinska Sielaff and Camilla Urbaniak and Ganesh Babu Malli Mohan and Victor G. Stepanov and Quyen Tran and Jason M. Wood and Jeremiah J. Minich and Daniel McDonald and Teresa Mayer and Rob Knight and Fathi Karouia and George E. Fox and Kasthuri Venkateswaran},
  journal={Microbiome},
  year={2019},
  volume={7}
}
BackgroundThe International Space Station (ISS) is a closed system inhabited by microorganisms originating from life support systems, cargo, and crew that are exposed to unique selective pressures such as microgravity. To date, mandatory microbial monitoring and observational studies of spacecraft and space stations have been conducted by traditional culture methods, although it is known that many microbes cannot be cultured with standard techniques. To fully appreciate the true number and… 
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References

SHOWING 1-10 OF 105 REFERENCES
Succession and persistence of microbial communities and antimicrobial resistance genes associated with International Space Station environmental surfaces
TLDR
There was an increase in AMR and virulence gene factors detected over the period sampled, and metagenome sequences of human pathogens persisted over time, andComparative analysis of the microbial compositions of ISS with Earth analogs revealed that the ISS environmental surfaces were different in microbial composition.
Four-year bacterial monitoring in the International Space Station—Japanese Experiment Module “Kibo” with culture-independent approach
TLDR
From the data on bacterial abundance and phylogenetic affiliation, Kibo has been microbiologically well maintained; however, the microbial community structure in Kibo may change with prolonged stay of astronauts, so it is suggested that bacterial cells are transferred to the surfaces of Kibo from the astronauts.
Characterization of Aspergillus fumigatus Isolates from Air and Surfaces of the International Space Station
TLDR
An initial characterization of two isolates, ISSFT-021 and IF1SW-F4, of Aspergillus fumigatus collected from the ISS and a comparison to the experimentally established clinical isolates Af293 and CEA10 found that both ISS isolates exhibited normal in vitro growth and chemical stress tolerance yet caused higher lethality in a vertebrate model of invasive disease.
Recurrent isolation of extremotolerant bacteria from the clean room where Phoenix spacecraft components were assembled.
TLDR
The microbial burden of the Phoenix spacecraft assembly environment was assessed in a systematic manner via several cultivation-based techniques and a suite of NASA-certified, cultivation-independent biomolecule-based detection assays and it was suggested that certain species may be better adapted to environmental conditions found during 2P and 3P.
Molecular bacterial community analysis of clean rooms where spacecraft are assembled.
TLDR
This study presents the first extended molecular survey and comparison of NASA spacecraft assembly facilities, and provides new insights into the bacterial diversity of clean room environments.
Microbiomes of the dust particles collected from the International Space Station and Spacecraft Assembly Facilities
TLDR
The results of this study provide strong evidence that specific human skin-associated microorganisms make a substantial contribution to the ISS microbiome, which is not the case in Earth-based cleanrooms.
A microbial survey of the International Space Station (ISS)
TLDR
The microbial community composition on the ISS was more similar to home surfaces than to the human microbiome samples, compared to those from both homes on Earth and data from the Human Microbiome Project.
New perspectives on viable microbial communities in low-biomass cleanroom environments
TLDR
The findings of the first ever comprehensive effort to assess the viability of cells in low-biomass environmental samples, and correlate differential viability with phylogenetic affiliation are presented.
Diversity of Bacterial Communities of Fitness Center Surfaces in a U.S. Metropolitan Area
TLDR
Investigation of the overall bacterial ecology of selected fitness centers in a metropolitan area utilizing culture-independent pyrosequencing of the 16S rRNA genes found Staphylococcus was found to be the most prevalent genus and underscores the need to increase public awareness on the importance of personal hygiene and sanitation for public gym users.
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