Characterization of the syringomycin synthetase gene cluster. A link between prokaryotic and eukaryotic peptide synthetases.

@article{Guenzi1998CharacterizationOT,
  title={Characterization of the syringomycin synthetase gene cluster. A link between prokaryotic and eukaryotic peptide synthetases.},
  author={Eric Guenzi and Giuliano Galli and Ingeborg Grgurina and Dennis C. Gross and Guido Grandi},
  journal={The Journal of biological chemistry},
  year={1998},
  volume={273 49},
  pages={
          32857-63
        }
}
With this work we have completed the characterization of the syringomycin synthetase gene cluster. In particular, by sequencing additional 28.5 kilobase pairs we show that the nine modules involved in the binding of the nine amino acids of syringomycin are localized on SyrB and SyrE, with SyrE carrying eight modules. The recombinant SyrB and the first and second modules of SyrE (SyrE1 and SyrE2) have been expressed in Escherichia coli and purified. The biochemical data indicate that SyrB binds… CONTINUE READING

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