Characterization of the proteolytic enzymes in the midgut of the European Cockchafer, Melolontha melolontha (Coleoptera: Scarabaeidae).

@article{Wagner2002CharacterizationOT,
  title={Characterization of the proteolytic enzymes in the midgut of the European Cockchafer, Melolontha melolontha (Coleoptera: Scarabaeidae).},
  author={Wolfgang Wagner and Frank M{\"o}hrlen and Wolfgang Schnetter},
  journal={Insect biochemistry and molecular biology},
  year={2002},
  volume={32 7},
  pages={803-14}
}
In previous studies we showed that the resistance of the European Cockchafer, Melolontha melolontha, towards the Scarab specific Cry8C toxin of Bacillus thuringiensis japonensis strain Buibui is due to the complexity of proteinases in the midgut of the pest insect. In this study these proteinases were identified and characterized using a combination of synthetic substrates and specific inhibitors in zymograms, activity blots, and photometric/fluorometric assays. In the midgut juice three… CONTINUE READING

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