Characterization of the promoter region of the mouse Xist gene.

  title={Characterization of the promoter region of the mouse Xist gene.},
  author={N Pillet and Christophe Bonny and Daniel F Schorderet},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={92 26},
The mouse Xist gene is expressed exclusively from the inactive X chromosome and may be implicated in initiating X inactivation. To better understand the mechanisms underlying the control of Xist expression, we investigated the upstream regulatory region of the mouse Xist promoter. A 1.2-kb upstream region of the Xist gene was sequenced and promoter activity was studied by chloramphenicol acetyltransferase (CAT) assays after transfection in murine XX and XY cell lines. The region analyzed (-1157… CONTINUE READING