Characterization of the prime and non-prime active site specificities of proteases by proteome-derived peptide libraries and tandem mass spectrometry

@article{Schilling2011CharacterizationOT,
  title={Characterization of the prime and non-prime active site specificities of proteases by proteome-derived peptide libraries and tandem mass spectrometry},
  author={Oliver Schilling and Pitter F. Huesgen and O. De La Barre and Ulrich Keller and Christopher M. Overall},
  journal={Nature Protocols},
  year={2011},
  volume={6},
  pages={111-120}
}
To link cleaved substrates in complex systems with a specific protease, the protease active site specificity is required. Proteomic identification of cleavage sites (PICS) simultaneously determines both the prime- and non-prime-side specificities of individual proteases through identification of hundreds of individual cleavage sequences from biologically relevant, proteome-derived peptide libraries. PICS also identifies subsite cooperativity. To generate PICS peptide libraries, cellular… CONTINUE READING
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