Characterization of the preprotein translocase of the outer mitochondrial membrane by blue native electrophoresis.

@article{Dekker1996CharacterizationOT,
  title={Characterization of the preprotein translocase of the outer mitochondrial membrane by blue native electrophoresis.},
  author={P. J. Dekker and Hannelore M{\"u}ller and Joachim Rassow and Nikolaus Pfanner},
  journal={Biological chemistry},
  year={1996},
  volume={377 7-8},
  pages={
          535-8
        }
}
The mitochondrial outer membrane contains import receptors for nuclear-encoded preproteins and a general import pore responsible for membrane translocation of preproteins. Receptors and the general import pore have been suggested to assemble into a loose complex. However, biochemical characterization of the complex has been limited so far. We report that blue native electrophoresis separates two complexes. One complex of approximately 400 kDa contains the receptor Tom22 and the general import… CONTINUE READING
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