Characterization of the pilF—pilD pilus‐assembly locus of Neisseria gonorrhoeae

  title={Characterization of the pilF—pilD pilus‐assembly locus of Neisseria gonorrhoeae},
  author={Nancy E. Freitag and H. Steven Seifert and Michael Koomey},
  journal={Molecular Microbiology},
Expression of Type IV pili by the bacterial pathogen Neisseria gonorrhoeae appears to be essential for colonization of the human host. Several N. gonorrhoeae gene products have been recently identified which bear homology to proteins involved in pilus assembly and protein export in other bacterial systems. We report here the isolation and characterization of transposon insertion mutants in N. gonorrhoeae whose phenotypes indicate that the N. gonorrhoeae pilF and pilD gene products are required… 

Charged tmRNA but not tmRNA‐mediated proteolysis is essential for Neisseria gonorrhoeae viability

It is reported that tmRNA is essential in Neisseria gonorrhoeae, and although tagging per se appears to be required for gonococcal viability, tagging for proteolysis does not, which suggests that the essential roles of tm RNA in N.gonor rhoeae may include resolving stalled translation complexes and/or preventing depletion of free ribosomes.

Differential interaction forces govern bacterial sorting in early biofilms

It is concluded that in early biofilms the density and rupture force of bacterial surface structures can trigger cell sorting based on similar physical principles as in developing embryos.

Pilin regulation in the pilT mutant of Neisseria gonorrhoeae strain MS11.

Levels of pilE transcripts and its deduced protein, the major Tfp subunit pilin, were increased most markedly by a mutation in pilT, and further studies revealed that pilE expression was also controlled by two other genes encoding Tfp biogenesis proteins, pilD and pilF.

Pilin regulation in thepilTmutantofNeisseria gonorrhoeae strainMS11

Pilin regulation in thepilTmutantofNeisseria gonorrhoeae strainMS11 is studied to determine the role of EMT in the regulation of IL-12 in gonorrrhoea infection.

Substitutions in the N‐terminal alpha helical spine of Neisseria gonorrhoeae pilin affect Type IV pilus assembly, dynamics and associated functions

The findings document for the first time in a defined genetic background the phenomenon of pilin intermolecular complementation in which assembly defective pilin can be rescued into purifiable Tfp by coexpression of wild‐type PilE.

The comP locus of Neisseria gonorrhoeae encodes a type IV prepilin that is dispensable for pilus biogenesis but essential for natural transformation

The process of DNA uptake in gonococci is now known to require the expression of at least three distinct components: Tfp, the recently identified PilT protein and ComP.

PilP, a pilus biogenesis lipoprotein in Neisseria gonorrhoeae, affects expression of PilQ as a high‐molecular‐mass multimer

The results suggest that PilP is required for PilQ function and that PilQ and PilC may interact during the terminal stages of pilus biogenesis, and support the hypothesis that the Gc PilQ multimer corresponds to a physiologically relevant form of the protein required for pilusBiogenesis.

Genetic Interactions of DNA Repair Pathways in the Pathogen Neisseria meningitidis

Different differences between N. meningitidis and established DNA repair characteristics in E. coli and other species were identified, and NER was identified as the main meningococcal defense pathway against UV-induced DNA damage.

The pilus and porin of Neisseria gonorrhoeae cooperatively induce Ca2+ transients in infected epithelial cells

Results strongly suggest that the pilus and porin cooperate to modulate calcium signalling in epithelial cells, and propose a model to explain how N. gonorrhoeae triggers Ca2+ transients in the initial stages of pilus‐mediated attachment.

Genetic Characterization of a New Type IV-A Pilus Gene Cluster Found in Both Classical and El Tor Biotypes ofVibrio cholerae

ABSTRACT The Vibrio cholerae genome contains a 5.4-kbpil gene cluster that resembles the Aeromonas hydrophila tap gene cluster and other type IV-A pilus assembly operons. The region consists of five