Characterization of the osteoclast vacuolar H(+)-ATPase B-subunit.

  title={Characterization of the osteoclast vacuolar H(+)-ATPase B-subunit.},
  author={Marcjanna Bartkiewicz and Natividad Hernando and Sakamuri V Reddy and Garson David Roodman and Rudi A. Baron},
  volume={160 2},
During bone resorption, osteoclasts acidify the extracellular bone resorbing compartment via a vacuolar H(+)-ATPase (V-ATPase), which resides in the ruffled-border membrane. In an effort to characterize the composition of the osteoclast V-ATPase catalytic domain, we have isolated a cDNA clone that encodes the V-ATPase B-subunit from a cDNA library constructed from highly purified chicken osteoclasts. Comparison of the predicted amino-acid sequence with the published sequences of isoforms of V… CONTINUE READING