Characterization of the non-competitive antagonist binding site of the NMDA receptor in dark Agouti rats.

@article{Sun2004CharacterizationOT,
  title={Characterization of the non-competitive antagonist binding site of the NMDA receptor in dark Agouti rats.},
  author={Wenlin Sun and William Dave Wessinger},
  journal={Life sciences},
  year={2004},
  volume={75 12},
  pages={1405-15}
}
The ability of non-competitive NMDA antagonists and other selected compounds to inhibit [3H]MK-801 binding to the NMDA receptor in brain membranes was evaluated in female, dark Agouti rats. In homologous competition binding studies the average apparent affinity (KD) of [3H]MK-801 for its binding site was 5.5 nM and the binding site density (Bmax) was 1.83 pmol/mg protein. Inhibition of [3H]MK-801 binding by non-competitive NMDA antagonists was best described with a one-site competition model… CONTINUE READING