Characterization of the nisin gene as part of a polycistronic operon in the chromosome of Lactococcus lactis ATCC 11454.

@article{Steen1991CharacterizationOT,
  title={Characterization of the nisin gene as part of a polycistronic operon in the chromosome of Lactococcus lactis ATCC 11454.},
  author={M. Steen and Y. J. Chung and J. N. Hansen},
  journal={Applied and environmental microbiology},
  year={1991},
  volume={57 4},
  pages={
          1181-8
        }
}
  • M. Steen, Y. J. Chung, J. N. Hansen
  • Published 1991
  • Biology, Medicine
  • Applied and environmental microbiology
  • The location and organization of the nisin locus in Lactococcus lactis ATCC 11454 were studied. Primer extension of in vivo mRNA transcripts of the gene that encodes the nisin prepropeptide sequence indicated the presence of a promoter at least 4 kb upstream from the nisin gene and that the mRNA has several processing sites. Restriction fragment patterns using rare-cutting enzymes, orthogonal pulsed-field clamped homogeneous electric field (CHEF) agarose gel electrophoresis, and hybridization… CONTINUE READING
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