Characterization of the mouse interleukin-5 promoter in a mouse TH2 T cell clone.


The proximal mouse IL-5 promoter was examined using a mouse TH2 clone stimulated through the T cell receptor using anti-CD3 monoclonal antibody. DNase I protection defined four protein binding regions [IL-5RE-A, -69/-45; -B, (-90/-76); -C, (-154/-130); and -D (-176/-157)]. Stimulation-dependent binding, which was seen in the IL-5RE-B, -D regions and the 5' end of tIL-5RE-A, did not require new protein synthesis inhibitor during cell activation. EMSA using probes targeted to the IL-5RE-B, -C, -D regions demonstrated the multimeric nature of the bound proteins. By transfection analysis using a series of truncated IL-5 promoter-luciferase constructs, IL-5RE-C and -D contributed little to constitutive or inducible activity. The CLE0 site in the IL-5RE-A region contributed to full transcriptional activity but was not sufficient to mediate full activity. Full stimulation-dependent activity required the IL-5RE-B region and/or the GATA site (-70/-60).

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@article{Stranick1998CharacterizationOT, title={Characterization of the mouse interleukin-5 promoter in a mouse TH2 T cell clone.}, author={Kimberley S. Stranick and Annette S Uss and Demetris N. Zambas and Robert W. Egan and M. Motasim Billah and Shelby P. Umland}, journal={Biochemical and biophysical research communications}, year={1998}, volume={252 1}, pages={56-62} }