Characterization of the mechanisms controlling Greatwall activity.

  title={Characterization of the mechanisms controlling Greatwall activity.},
  author={Suzanne Vigneron and Aicha Gharbi-Ayachi and Anne-Aur{\'e}lie Raymond and Andrew Burgess and Jean-Claude Labb{\'e} and Gilles Labesse and Bernard Monsarrat and Thierry Lorca and Anna Maria Castro},
  journal={Molecular and cellular biology},
  volume={31 11},
Here we investigate the mechanisms regulating Greatwall (Gwl), a serine/threonine kinase essential for promoting the correct timing of mitosis. We identify Gwl as a unique AGC kinase that, unlike most AGC members, appears to be devoid of a hydrophobic motif despite the presence of a functional hydrophobic pocket. Our results suggest that Gwl activation could be mediated by the binding of its hydrophobic pocket to the hydrophobic motif of another AGC kinase. Our molecular modeling and mutagenic… CONTINUE READING