Characterization of the mUBC9-binding sites required for E2A protein degradation.

  title={Characterization of the mUBC9-binding sites required for E2A protein degradation.},
  author={Gordon S. Huggins and Michael T. Chin and Nicholas E. S. Sibinga and Sandra L. Lee and Edgar Haber and Margaret E. Lee},
  journal={The Journal of biological chemistry},
  volume={274 40},
Mammalian Ubc9 (mUbc9) is required for rapid degradation of the E2A proteins E12 and E47 by the ubiquitin-proteasome system. We have shown elsewhere that mUbc9 interacts with amino acids 477-530 of E12/E47. Here we test the hypothesis that this region, rich in proline, glutamic acid, serine, and threonine (PEST) residues, serves as the E2A protein degradation domain (DD). An E2A protein lacking this region, E47Delta(478-531), was significantly more stable than wild-type E47(half-life of more… CONTINUE READING