Characterization of the leptospiral outer membrane and description of three novel leptospiral membrane proteins.

@article{Haake2002CharacterizationOT,
  title={Characterization of the leptospiral outer membrane and description of three novel leptospiral membrane proteins.},
  author={David A. Haake and James Matsunaga},
  journal={Infection and immunity},
  year={2002},
  volume={70 9},
  pages={4936-45}
}
The outer membrane (OM) of the mammalian pathogen Leptospira kirschneri was isolated in the form of membrane vesicles by alkaline plasmolysis and separated from the protoplasmic cylinder by sucrose density gradient ultracentrifugation. All four components of the alkaline plasmolysis buffer, including 1.0 M NaCl, 27% sucrose (wt/vol), 2 mM EDTA, and 10 mM Tris (pH 9), were required for efficient OM release, as judged by recovery of leptospiral lipopolysaccharide. Two populations of OM vesicles… CONTINUE READING

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