Characterization of the interaction of plant transcription factors using a bacterial repressor protein.

@article{Frohberg1991CharacterizationOT,
  title={Characterization of the interaction of plant transcription factors using a bacterial repressor protein.},
  author={Claus Frohberg and Lisa Heins and Christiane Gatz},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1991},
  volume={88 23},
  pages={10470-4}
}
Transcription initiation from a eukaryotic polymerase II promoter requires a functional interaction of regulatory transcriptional activators with at least one of the basal transcription factors binding in the vicinity of the TATA box. To characterize this type of interaction in vivo, we have inserted the bacterial Tet repressor-operator complex in nine different positions between an enhancer element (as-1) and the TATA box of the cauliflower mosaic virus (CaMV) 35S RNA promoter. A direct… CONTINUE READING