Characterization of the interaction between the baculovirus replication factors LEF-1 and LEF-2.

@article{Evans1997CharacterizationOT,
  title={Characterization of the interaction between the baculovirus replication factors LEF-1 and LEF-2.},
  author={J T Evans and Douglas J. Leisy and George F. Rohrmann},
  journal={Journal of virology},
  year={1997},
  volume={71 4},
  pages={3114-9}
}
The Autographa californica multinucleocapsid nuclear polyhedrosis virus has six genes required and three genes stimulatory for transient DNA replication. We demonstrate that the products of two of these genes, LEF-1 and LEF-2, interact in both two-hybrid assays using Saccharomyces cerevisiae and glutathione S-transferase fusion affinity assays. Using yeast-two-hybrid assays, we mapped the interaction domain of LEF-2 to amino acids between positions 20 and 60. Extensive deletion analyses of LEF… CONTINUE READING
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