• Biology, Medicine
  • Published in
    The Journal of biological…
    1983

Characterization of the insulin receptor kinase purified from human placental membranes.

@article{Kasuga1983CharacterizationOT,
  title={Characterization of the insulin receptor kinase purified from human placental membranes.},
  author={Masato Kasuga and Yoko Fujita-Yamaguchi and D L Blithe and Morris F. White and C. Ronald Kahn},
  journal={The Journal of biological chemistry},
  year={1983},
  volume={258 18},
  pages={
          10973-80
        }
}
The insulin receptor purified from human placenta by sequential affinity chromatography on wheat germ agglutinin- and insulin-Sepharose to near homogeneity retained tyrosine-specific protein kinase activity. This purified insulin receptor kinase specifically catalyzed the incorporation of 32P from [gamma-32P]ATP into not only the beta-subunit of the insulin receptor but also histone H2B, a synthetic peptide which is sequentially similar to the site of tyrosine phosphorylation in pp60src (a gene… CONTINUE READING

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