Characterization of the inducible nickel and cobalt resistance determinant cnr from pMOL28 of Alcaligenes eutrophus CH34

@article{Liesegang1993CharacterizationOT,
  title={Characterization of the inducible nickel and cobalt resistance determinant cnr from pMOL28 of Alcaligenes eutrophus CH34},
  author={H. Liesegang and K. Lemke and R. A. Siddiqui and H. Schlegel},
  journal={Journal of Bacteriology},
  year={1993},
  volume={175},
  pages={767 - 778}
}
From pMOL28, one of the two heavy metal resistance plasmids of Alcaligenes eutrophus strain CH34, we cloned an EcoRI-PstI fragment into plasmid pVDZ'2. This hybrid plasmid conferred inducible nickel and cobalt resistance (cnr) in two distinct plasmid-free A. eutrophus hosts, strains AE104 and H16. Resistances were not expressed in Escherichia coli. The nucleotide sequence of the 8.5-kb EcoRI-PstI fragment (8,528 bp) revealed seven open reading frames; two of these, cnrB and cnrA, were assigned… Expand
Regulation of the cnr Cobalt and Nickel Resistance Determinant of Ralstonia eutropha (Alcaligenes eutrophus) CH34
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The linked resistance to nickel and cobalt of Ralstonia eutropha-like strain CH34 is encoded by the cnr operon, which is localized on the megaplasmid pMOL28, and transcriptional fusions were constructed between fragments of cnrYXH and the luxCDABE, luciferase reporter genes. Expand
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Alcaligenes eutrophus CH34 is a facultative chemolithotroph with plasmid-bound resistance to heavy metals
Alcaligenes eutrophus strain CH34, which was isolated as a bacterium resistant to cobalt, zinc, and cadmium ions, shares with A. eutrophus strain H16 the ability to grow lithoautotrophically onExpand
Plasmid pMOL28-mediated inducible nickel resistance in Alcaligenes eutrophus strain CH34
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