Characterization of the human zona pellucida from fertilized and unfertilized eggs.

@article{Shabanowitz1988CharacterizationOT,
  title={Characterization of the human zona pellucida from fertilized and unfertilized eggs.},
  author={Robert B. Shabanowitz and Michael G O'Rand},
  journal={Journal of reproduction and fertility},
  year={1988},
  volume={82 1},
  pages={
          151-61
        }
}
Zonae pellucidae were isolated from a variety of human eggs collected from follicular aspirates for in-vitro fertilization. Zonae were removed from pools of eggs classified as fertilized but unsuitable for embryo transfer, inseminated but not fertilized, and immature and not inseminated. Isolated zonae were heat solubilized, iodinated and separated by two-dimensional electrophoresis. Under reducing conditions, zonae from unfertilized eggs separated into three acidic proteins with molecular… CONTINUE READING
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