Characterization of the human heart mitochondrial proteome

@article{Taylor2003CharacterizationOT,
  title={Characterization of the human heart mitochondrial proteome},
  author={Steven W. Taylor and E. Fahy and Bing Zhang and G. M. Glenn and D. Warnock and S. Wiley and A. Murphy and S. Gaucher and R. Capaldi and B. Gibson and Soumitra S Ghosh},
  journal={Nature Biotechnology},
  year={2003},
  volume={21},
  pages={281-286}
}
To gain a better understanding of the critical role of mitochondria in cell function, we have compiled an extensive catalogue of the mitochondrial proteome using highly purified mitochondria from normal human heart tissue. Sucrose gradient centrifugation was employed to partially resolve protein complexes whose individual protein components were separated by one-dimensional PAGE. Total in-gel processing and subsequent detection by mass spectrometry and rigorous bioinformatic analysis yielded a… Expand

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