Characterization of the guinea pig cytomegalovirus genome by molecular cloning and physical mapping.

@article{Gao1984CharacterizationOT,
  title={Characterization of the guinea pig cytomegalovirus genome by molecular cloning and physical mapping.},
  author={Minggeng Gao and Harriet C. Isom},
  journal={Journal of virology},
  year={1984},
  volume={52 2},
  pages={436-47}
}
Fragments of guinea pig cytomegalovirus (GPCMV) DNA produced by HindIII or EcoRI restriction endonuclease digestion were cloned into vectors pBR322 and pACYC184, and recombinant fragments representing ca. 97% of the genome were constructed. Hybridization of 32P-labeled cloned and gel-purified HindIII, EcoRI, and XbaI fragments to Southern blots of HindIII-, EcoRI-, and XbaI-cleaved GPCMV DNA verified the viral origin of cloned fragments and allowed construction of HindIII, EcoRI, and XbaI… CONTINUE READING

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