Characterization of the genetic elements required for site-specific integration of plasmid pSE211 in Saccharopolyspora erythraea.

Abstract

The 18.1-kilobase plasmid pSE211 integrates into the chromosome of Saccharopolyspora erythraea at a specific attB site. Restriction analysis of the integrated plasmid, pSE211int, and adjacent chromosomal sequences allowed identification of attP, the plasmid attachment site. Nucleotide sequencing of attP, attB, attL, and attR revealed a 57-base-pair sequence… (More)

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@article{Brown1990CharacterizationOT, title={Characterization of the genetic elements required for site-specific integration of plasmid pSE211 in Saccharopolyspora erythraea.}, author={D. P. Brown and Kenneth B. Idler and L. Katz}, journal={Journal of bacteriology}, year={1990}, volume={172 4}, pages={1877-88} }