Characterization of the genetic components of Streptomyces lividans linear plasmid SLP2 for replication in circular and linear modes.

@article{Xu2006CharacterizationOT,
  title={Characterization of the genetic components of Streptomyces lividans linear plasmid SLP2 for replication in circular and linear modes.},
  author={Mingxuan Xu and Yingmin Zhu and Ran Zhang and Meijuan Shen and Weihong Jiang and Guo-Ping Zhao and Zhongjun Qin},
  journal={Journal of bacteriology},
  year={2006},
  volume={188 19},
  pages={6851-7}
}
The nucleotide sequence of Streptomyces lividans linear plasmid SLP2 consists of 50,410 bp (C. H. Huang, C. Y. Chen, H. H. Tsai, C. Chen, Y. S. Lin, and C. W. Chen, Mol. Microbiol. 47:1563-1576, 2003). Here we report that the basic SLP2 locus for plasmid replication in circular mode resembles that of Streptomyces linear plasmids pSLA2 and SCP1 and comprises iterons(SLP2) and the adjacent rep(SLP2) gene. More efficient replication additionally required the 47-bp sequence between bp 581 and 628… CONTINUE READING

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