Characterization of the gene for factor C, an extracellular signal protein involved in morphological differentiation of Streptomyces griseus.

Abstract

The gene encoding factor C (facC), an extracellular signal protein involved in cellular differentiation, was cloned from Streptomyces griseus 45H, and the complete nucleotide sequence was determined. The deduced amino acid sequence was confirmed by HPLC/electrospray ionization-mass spectrometry analysis. The full-length protein consists of 324 amino acids and has a predicted molecular mass of 34,523 Da. The mature extracellular 286 amino acid protein (31,038 Da) is probably produced by cleaving off a 38 amino acid secretion signal sequence. Southern hybridization detected facC in several other Streptomyces strains, but database searches failed to identify a protein with significant homology to factor C. Expression of facC from a low-copy-number vector in S. griseus 52-1 resulted in a phenotypic effect similar to that given by exogenously added factor C protein.

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@article{Birk1999CharacterizationOT, title={Characterization of the gene for factor C, an extracellular signal protein involved in morphological differentiation of Streptomyces griseus.}, author={Zsuzsanna Birk{\'o} and Andrea S{\"{u}megi and A Vinnai and Gilles P van Wezel and Ferenc Szesz{\'a}k and S. Vit{\'a}lis and P{\'a}l Tam{\'a}s Szab{\'o} and Zolt{\'a}n Kele and Tam{\'a}s Jan{\'a}ky and S{\'a}ndor B{\'i}r{\'o}}, journal={Microbiology}, year={1999}, volume={145 ( Pt 9)}, pages={2245-53} }