Characterization of the gene for factor C, an extracellular signal protein involved in morphological differentiation of Streptomyces griseus.

Abstract

The gene encoding factor C (facC), an extracellular signal protein involved in cellular differentiation, was cloned from Streptomyces griseus 45H, and the complete nucleotide sequence was determined. The deduced amino acid sequence was confirmed by HPLC/electrospray ionization-mass spectrometry analysis. The full-length protein consists of 324 amino acids and has a predicted molecular mass of 34,523 Da. The mature extracellular 286 amino acid protein (31,038 Da) is probably produced by cleaving off a 38 amino acid secretion signal sequence. Southern hybridization detected facC in several other Streptomyces strains, but database searches failed to identify a protein with significant homology to factor C. Expression of facC from a low-copy-number vector in S. griseus 52-1 resulted in a phenotypic effect similar to that given by exogenously added factor C protein.

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Showing 1-10 of 25 references

Received

  • 1999

Amino acid sequence homology of factor C produced by Streptomyces griseus with regulatory proteins of zinc finger type

  • F Szesza ! K, S Vita ! Lis, Biro, S Dalmi
  • 1997

Developmental heterogeneity of Sterptomyces griseus conidia

  • Szabo, G Vita ! Lis, S Szesza ! K, F Biro
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Presence of factor C in streptomycetes and other bacteria

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Factor C, a regulatory protein of Streptomyces griseus, induces release of potassium from the mycelium

  • F Szesza ! K, S Vita ! Lis, Szabo
  • 1989

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