Characterization of the early events leading to totipotency in an Arabidopsis protoplast liquid culture by temporal transcript profiling.

@article{Chupeau2013CharacterizationOT,
  title={Characterization of the early events leading to totipotency in an Arabidopsis protoplast liquid culture by temporal transcript profiling.},
  author={Marie-christine Chupeau and Fabienne Granier and Olivier Pichon and Jean-Pierre Renou and Val{\'e}rie Gaudin and Yves Chupeau},
  journal={The Plant cell},
  year={2013},
  volume={25 7},
  pages={2444-63}
}
The molecular mechanisms underlying plant cell totipotency are largely unknown. Here, we present a protocol for the efficient regeneration of plants from Arabidopsis thaliana protoplasts. The specific liquid medium used in our study leads to a high rate of reentry into the cell cycle of most cell types, providing a powerful system to study dedifferentiation/regeneration processes in independent somatic cells. To identify the early events in the establishment of totipotency, we monitored the… CONTINUE READING

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