Characterization of the currents induced by sigma ligands in NCB20 neuroblastoma cells

@article{Morio1994CharacterizationOT,
  title={Characterization of the currents induced by sigma ligands in NCB20 neuroblastoma cells},
  author={Yasunori Morio and Hayato Tanimoto and Takashi Yakushiji and Yasuto Morimoto},
  journal={Brain Research},
  year={1994},
  volume={637},
  pages={190-196}
}
Electrical and pharmacological properties of currents induced by compounds having affinities for putative sigma receptors were investigated with NCB20 cells by use of the whole-cell patch-clamp technique. Antipsychotics and naloxone induced inward currents with a decrease in membrane conductance at a holding potential of -60 mV. The rank order of potency for compounds inducing these currents was bromperidol > haloperidol > mosapramine = clocapramine > carpipramine > chlorpromazine > remoxipride… Expand
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Electrophysiological and binding studies on intact NCB-20 cells suggest presence of a low affinity sigma receptor.
TLDR
The high affinity site was similar pharmacologically to the sigma receptor assayed in membrane fragments from NCB-20 cells, however, the low affinity site showed a slightly different profile, highlighted by a reverse stereoselectivity. Expand
Calcium-dependent displacement of haloperidol-sensitive σ receptor binding in rat hippocampal slices following tissues depolarization
TLDR
The results suggest that a calcium-dependent, depolarization-induced reduction in sigma receptor binding may have been caused by the release of an endogenous sigma ligand in rat hippocampal tissue. Expand
Inhibition of potassium currents by the sigma receptor ligand (+)-3-(3-hydroxyphenyl)-N-(1-propyl)piperidine in sympathetic neurons of the mouse isolated hypogastric ganglion
The actions of (+)-3-(3-hydroxyphenyl)-N-(1-propyl)piperidine [(+)3-PPP] on sympathetic neurons of the mouse isolated hypogastric ganglion were studied using the current clamp and single electrodeExpand
Evidence for sigma opioid receptor: binding of [3H]SKF-10047 to etorphine-inaccessible sites in guinea-pig brain.
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TLDR
It is suggested that EI sites are not mu receptors but may represent sigma receptors in the central nervous system, mediating psychotomimetic effects of several opioids and other drugs. Expand
Serotonin receptor-mediated activation of adenylate cyclase in the neuroblastoma NCB.20: a novel 5-hydroxytryptamine receptor.
TLDR
Evidence is presented indicating that serotonin stimulation of adenylate cyclase is mediated by receptors that are distinct from other positively coupled receptors (beta-adrenergic, histamine, and prostacyclin), and the results point to a novel serotonin receptor which has a low density in these cells. Expand
Effects of iminodibenzyl antipsychotic drugs on cerebral dopamine and α-adrenergic receptors
Abstract The iminodibenzyl antipsychotic drugs, clocapramine, carpipramine and Y-516 were studied in order to elucidate their mechanisms of action. They all accelerated the accumulation of theExpand
The effects of morphine- and nalorphine- like drugs in the nondependent and morphine-dependent chronic spinal dog.
TLDR
It has been shown that buprenorphine is a partial agonist of the mu type which both suppressed and precipitated abstinence in the morphine-dependent dog while morphine and propoxyphene are stronger agonists. Expand
Ethanol potentiation of 5-HT3 receptor-mediated ion current in NCB-20 neuroblastoma cells
TLDR
The effect of acute ethanol (EtOH) exposure on 5-HT3 receptor-mediated ion current was examined in whole-cell patch-clamp recordings from NCB-20 neuroblastoma cells and indicated that it was mediated by 5- HT3 receptors. Expand
Membrane current responses of NG108‐15 mouse neuroblastoma x rat glioma hybrid cells to bradykinin.
TLDR
It is concluded that the dual response to BK results from opposing effects on two different species of K+ current, probably mediated by a transient rise in intracellular Ca2+. Expand
Multiple vascular effects of sigma and PCP ligands: inhibition of amine uptake and contractile responses.
TLDR
The lack of selectivity of many sigma and PCP ligands, significant effects on norepinephrine uptake, as well as the potential utility of the rat tail artery to explore the functional properties of these ligands are demonstrated. Expand
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