Characterization of the conjugation system associated with the Staphylococcus aureus plasmid pJE1.

@article{Evans1988CharacterizationOT,
  title={Characterization of the conjugation system associated with the Staphylococcus aureus plasmid pJE1.},
  author={John Grimley Evans and Keith G. H. Dyke},
  journal={Journal of general microbiology},
  year={1988},
  volume={134 1},
  pages={1-8}
}
The conjugation system of Staphylococcus aureus that is conferred, at least in part, by plasmid pJE1, requires cell-to-cell contact. Optimum transfer was found when the numbers of donor and recipient cells were equal. Certain antibiotics increased the conjugation frequency. Fragments of plasmid pJE1 were cloned into a staphylococcal plasmid vector; although separate clones were isolated that conferred ethidium bromide resistance and gentamicin resistance, none of the clones carried the ability… CONTINUE READING

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