Characterization of the binding of amphotericin B to Saccharomyces cerevisiae and relationship to the antifungal effects.

Abstract

Based on the enhanced fluorescence of amphotericin B in acid solutions, a quantitative assay for this polyene antibiotic has been developed that is sensitive and linear in the range of 0.1 to 10.0 muM. The binding of amphotericin B to Saccharomyces cerevisiae was assayed under various conditions as the amount bound to cells in a dialysis chamber or after… (More)

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@article{KOTLERBRAJTBURG1974CharacterizationOT, title={Characterization of the binding of amphotericin B to Saccharomyces cerevisiae and relationship to the antifungal effects.}, author={JANINA KOTLER-BRAJTBURG and Gerald Medoff and David Schlessinger and George S. Kobayashi}, journal={Antimicrobial agents and chemotherapy}, year={1974}, volume={6 6}, pages={770-6} }