Characterization of the amino-terminal transcriptional activation function of the human estrogen receptor in animal and yeast cells.

@article{Metzger1995CharacterizationOT,
  title={Characterization of the amino-terminal transcriptional activation function of the human estrogen receptor in animal and yeast cells.},
  author={Daniel Metzger and Simak Ali and J M Bornert and Pierre Chambon},
  journal={The Journal of biological chemistry},
  year={1995},
  volume={270 16},
  pages={9535-42}
}
We have previously reported that the transcriptional activation function AF-1, located in the A/B region of the human estrogen receptor, exhibits cell-type and promoter context specificity in both animal cells and yeast. To further characterize AF-1, we have constructed a number of deletion mutants spanning the A/B region in the context of either the whole human estrogen receptor or the A/B region linked to the GAL4 DNA binding domain, and tested their transcriptional activity in chicken embryo… CONTINUE READING