Characterization of the activities of the CpeY, CpeZ, and CpeS bilin lyases in phycoerythrin biosynthesis in Fremyella diplosiphon strain UTEX 481.

@article{Biswas2011CharacterizationOT,
  title={Characterization of the activities of the CpeY, CpeZ, and CpeS bilin lyases in phycoerythrin biosynthesis in Fremyella diplosiphon strain UTEX 481.},
  author={Avijit Biswas and Mohamed Nazim Boutaghou and Richard M. Alvey and Christina M. Kronfel and Richard B Cole and Donald A Bryant and Wendy M. Schluchter},
  journal={The Journal of biological chemistry},
  year={2011},
  volume={286 41},
  pages={35509-21}
}
When grown in green light, Fremyella diplosiphon strain UTEX 481 produces the red-colored protein phycoerythrin (PE) to maximize photosynthetic light harvesting. PE is composed of two subunits, CpeA and CpeB, which carry two and three phycoerythrobilin (PEB) chromophores, respectively, that are attached to specific Cys residues via thioether linkages. Specific bilin lyases are hypothesized to catalyze each PEB ligation. Using a heterologous, coexpression system in Escherichia coli, the PEB… CONTINUE READING