Characterization of the TPX2 domains involved in microtubule nucleation and spindle assembly in Xenopus egg extracts.

@article{Brunet2004CharacterizationOT,
  title={Characterization of the TPX2 domains involved in microtubule nucleation and spindle assembly in Xenopus egg extracts.},
  author={St{\'e}phane Brunet and Teresa Sard{\'o}n and Timo Zimmerman and Torsten Wittmann and Rainer Pepperkok and Eric Karsenti and Isabelle Vernos},
  journal={Molecular biology of the cell},
  year={2004},
  volume={15 12},
  pages={
          5318-28
        }
}
TPX2 has multiple functions during mitosis, including microtubule nucleation around the chromosomes and the targeting of Xklp2 and Aurora A to the spindle. We have performed a detailed domain functional analysis of TPX2 and found that a large N-terminal domain containing the Aurora A binding peptide interacts directly with and nucleates microtubules in pure tubulin solutions. However, it cannot substitute the endogenous TPX2 to support microtubule nucleation in response to Ran guanosine… CONTINUE READING
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