Characterization of the Signal That Directs Tom20 to the Mitochondrial Outer Membrane

@article{Kanaji2000CharacterizationOT,
  title={Characterization of the Signal That Directs Tom20 to the Mitochondrial Outer Membrane},
  author={Sachiko Kanaji and Jun Iwahashi and Yuichiro Kida and Masao Sakaguchi and Katsuyoshi Mihara},
  journal={The Journal of Cell Biology},
  year={2000},
  volume={151},
  pages={277 - 288}
}
Tom20 is a major receptor of the mitochondrial preprotein translocation system and is bound to the outer membrane through the NH(2)-terminal transmembrane domain (TMD) in an Nin-Ccyt orientation. We analyzed the mitochondria-targeting signal of rat Tom20 (rTom20) in COS-7 cells, using green fluorescent protein (GFP) as the reporter by systematically introducing deletions or mutations into the TMD or the flanking regions. Moderate TMD hydrophobicity and a net positive charge within five residues… CONTINUE READING