Characterization of the RNA polymerase II and III complexes in Leishmania major.

@article{MartnezCalvillo2007CharacterizationOT,
  title={Characterization of the RNA polymerase II and III complexes in Leishmania major.},
  author={Santiago Mart{\'i}nez-Calvillo and Alka Saxena and Amanda Green and Aaron Leland and Peter J. Myler},
  journal={International journal for parasitology},
  year={2007},
  volume={37 5},
  pages={491-502}
}
Transcription of protein-coding genes in Leishmania major and other trypanosomatids differs from that in most eukaryotes and bioinformatic analyses have failed to identify several components of the RNA polymerase (RNAP) complexes. To increase our knowledge about this basic cellular process, we used tandem affinity purification (TAP) to identify subunits of RNAP II and III. Mass spectrometric analysis of the complexes co-purified with TAP-tagged LmRPB2 (encoded by LmjF31.0160) identified seven… CONTINUE READING
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