Characterization of the P450 monooxygenase NysL, responsible for C-10 hydroxylation during biosynthesis of the polyene macrolide antibiotic nystatin in Streptomyces noursei.

@article{Volokhan2006CharacterizationOT,
  title={Characterization of the P450 monooxygenase NysL, responsible for C-10 hydroxylation during biosynthesis of the polyene macrolide antibiotic nystatin in Streptomyces noursei.},
  author={Olga Volokhan and H{\aa}vard Sletta and Trond E. Ellingsen and Sergey B Zotchev},
  journal={Applied and environmental microbiology},
  year={2006},
  volume={72 4},
  pages={2514-9}
}
The nysL gene, encoding a putative P450 monooxygenase, was identified in the nystatin biosynthetic gene cluster of Streptomyces noursei. Although it has been proposed that NysL is responsible for hydroxylation of the nystatin precursor, experimental evidence for this activity was lacking. The nysL gene was inactivated in S. noursei by gene replacement, and the resulting mutant was shown to produce 10-deoxynystatin. Purification and an in vitro activity assay for 10-deoxynystatin demonstrated… CONTINUE READING

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Biosynthesis of the polyene macrolide antibiotic nystatin in Streptomyces noursei

  • E. Fjaervik, S. B. Zotchev
  • Appl. Microbiol. Biotechnol
  • 2005

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