Characterization of the Nicotiana tabacum L. genome by molecular cytogenetics

  title={Characterization of the Nicotiana tabacum L. genome by molecular cytogenetics},
  author={A. Y. Kenton and Alexander S Parokonny and Yuri Gleba and Michael D. Bennett},
  journal={Molecular and General Genetics MGG},
Nicotiana tabacum (2n=48) is a natural amphidiploid with component genomes S and T. We used non-radioactive in situ hybridization to provide physical chromosome markers for N. tabacum, and to determine the extant species most similar to the S and T genomes. Chromosomes of the S genome hybridized strongly to biotinylated total DNA from N. sylvestris, and showed the same physical localization of a tandemly repeated DNA sequence, HRS 60.1, confirming the close relationship between the S genome and… 

Evolutionary Implications of Genome and Karyotype Restructuring in Nicotiana tabacum L.

Progress in the understanding of the divergence of N. tabacum subsequent to its formation is reviewed and possible mechanisms that may have stimulated genetic changes are described, which can lead to enhanced fertility, more regular chromosome pairing, and the evolution of disomic inheritance.

Tobacco karyotyping by accurate centromere identification and novel repetitive DNA localization

The combination of immunostaining with FISH and GISH is critical to accurately karyotype tobacco and revealed novel intergenomic chromosome rearrangements and B-chromosome-like minichromosomes.

Chromosomal location of endogenous geminivirus-related DNA sequences inNicotiana tabacum L.

The N. tabacum (tobacco) nuclear genome carries approximately 25 multiple direct repeats of a geminivirus-related DNA (GRD), which provides an additional marker for the small chromosomes of the T genome and a useful phylogenetic tool.

COSII genetic maps of two diploid Nicotiana species provide a detailed picture of synteny with tomato and insights into chromosome evolution in tetraploid N. tabacum

These COSII and SSR markers link the cultivated tobacco map to those of wild diploid Nicotiana species and tomato, thus providing a platform for cross-reference of genetic and genomic information among them as well as other solanaceous species including potato, eggplant, pepper and the closely allied coffee.

Preferential elimination of repeated DNA sequences from the paternal, Nicotiana tomentosiformis genome donor of a synthetic, allotetraploid tobacco.

The data demonstrate that genetic changes in synthetic tobacco were fast, targeted to the paternal N. tomentosiformis-donated genome, and some of the changes showed concordance with changes that presumably occurred during evolution of natural tobacco.

Long-term genome diploidization in allopolyploid Nicotiana section Repandae (Solanaceae).

Sequence analysis indicates that 35S and 5S units most closely resemble maternal and paternal progenitors, respectively, in recent Nicotiana allopolyploid section Repandae.

Comparative genomics and repetitive sequence divergence in the species of diploid Nicotiana section Alatae.

This work reconstructs the history of karyotype and tandem repeat evolution in species of diploid Nicotiana section Alatae and suggests that sequence homogenization has replaced HRS60 family repeats at sub-telomeric regions, but that this process may not occur, or occurs more slowly, when the repeats are found at intercalary locations.

Repetitive DNA Dynamics and Polyploidization in the Genus Nicotiana (Solanaceae)

The lag-phase hypothesis, which describes post-polyploid radiations, is posed as a potentially important mechanism of evolution in Nicotiana section Suaveolentes, the largest polyploid section that consists of over half the current species diversity.

Chromosomal Localization of 5S and 18S rDNA in Eight Nicotiana Species and the Implications for Genome Evolution of Genus Nicotiana

The present results could provide obvious references for studies on the system and phylogenesis of genus Nicotiana and were of significance to genetic breeding and germplasm innovation for tobacco.

Species-specific evolution of telomeric and rDNA repeats in the tobacco composite genome

The results provide evidence for a species-specific evolution of telomeric and ribosomal repeats in the tobacco composite genome, and possible interactions between parental genomes in the composite genome of Nicotiana tabacum.



Comparison of the mitochondrial genome of Nicotiana tabacum with its progenitor species

Results indicate that the mitochondrial DNA of N. tabacum was inherited from N. sylvestris, and pattern variations indicate that extensive rearrangement of mtDNA has occurred in the evolution of these Nicotiana species.

Genomic in situ hybridization reveals the allopolyploid nature ofMilium montianum (Gramineae)

Genomic in situ hybridization is a potentially powerful tool for studying genome evolution and biosystematics and will often be useful for investigating the origins of wild and cultivated polyploid plant species, especially where conventional methods have failed.


The present publication is concerned with amphiploids between N .

Absence of some truncated genes in the amphidiploid Nicotiana tabacum.

Origin of Nicotiana tabacum L. detected by polypeptide composition of Fraction I protein

It is reported that N. tabacum arose from the hybridisation of N. sylvestris and N. tomentosiformis, and the putative progenitor species was N. otophora, a species in the Tomentosae section of Nicotiana.

A BamHI family of highly repeated DNA sequences of Nicotiana tabacum

Results suggest a tandem arrangement of this DNA repeat unit, which comprised about 2% of the nuclear genome of N. tabacum, and computer comparisons with other tandem plant-repeated DNA sequences could not detect any other homologous sequence.

Genomic divergence of allopatric sibling species studied by molecular cytogenetics of their F1hybrids

The ability to discriminate rapidly and reliably between the chromosomes of close relatives with almost identical karyotypes makes GISH invaluable in preliminary studies of phytogeny and confirms the sensitivity of the technique and demonstrates its potential use in evolutionary cytogenetics.


  • S. J. Sheen
  • Biology
    Evolution; international journal of organic evolution
  • 1972
The evolutionary process of N. tabacum may be clarified if synthesis of this species can be achieved through experimental hybridization and amphiploidy, and recently, Cameron (1965) added evidence that N. sylvestris contributed cytoplasm to the hybrid from which N.tabacum was evolved.

Tobacco single-copy DNA is highly homologous to sequences present in the genomes of its diploid progenitors

The results show that significant single-copy DNA sequence divergence has occurred between the diploid N. sylvestris and N. tomentosiformis genomes, but by applying the experimental criteria these single- copy DNAs are indistinguishable from their counterparts in the hybrid N. tabacum nucleus.

Nuclear DNA changes, genome differentiation and evolution inNicotiana (Solanaceae)

  • R. Narayan
  • Biology
    Plant Systematics and Evolution
  • 2004
A survey of 51 species fromNicotiana subgg.Tabacum, Rustica andPetunioides has shown that evolution was accompanied by a five-fold variation in nuclear DNA amounts. This variation, however, was not