Characterization of the Nicotiana tabacum L. genome by molecular cytogenetics

  title={Characterization of the Nicotiana tabacum L. genome by molecular cytogenetics},
  author={A. Y. Kenton and Alexander S Parokonny and Yuri Gleba and Michael D. Bennett},
  journal={Molecular and General Genetics MGG},
Nicotiana tabacum (2n=48) is a natural amphidiploid with component genomes S and T. We used non-radioactive in situ hybridization to provide physical chromosome markers for N. tabacum, and to determine the extant species most similar to the S and T genomes. Chromosomes of the S genome hybridized strongly to biotinylated total DNA from N. sylvestris, and showed the same physical localization of a tandemly repeated DNA sequence, HRS 60.1, confirming the close relationship between the S genome and… 
Evolutionary Implications of Genome and Karyotype Restructuring in Nicotiana tabacum L.
Progress in the understanding of the divergence of N. tabacum subsequent to its formation is reviewed and possible mechanisms that may have stimulated genetic changes are described, which can lead to enhanced fertility, more regular chromosome pairing, and the evolution of disomic inheritance.
Tobacco karyotyping by accurate centromere identification and novel repetitive DNA localization
The combination of immunostaining with FISH and GISH is critical to accurately karyotype tobacco and revealed novel intergenomic chromosome rearrangements and B-chromosome-like minichromosomes.
Chromosomal location of endogenous geminivirus-related DNA sequences inNicotiana tabacum L.
The N. tabacum (tobacco) nuclear genome carries approximately 25 multiple direct repeats of a geminivirus-related DNA (GRD), which provides an additional marker for the small chromosomes of the T genome and a useful phylogenetic tool.
COSII genetic maps of two diploid Nicotiana species provide a detailed picture of synteny with tomato and insights into chromosome evolution in tetraploid N. tabacum
These COSII and SSR markers link the cultivated tobacco map to those of wild diploid Nicotiana species and tomato, thus providing a platform for cross-reference of genetic and genomic information among them as well as other solanaceous species including potato, eggplant, pepper and the closely allied coffee.
Preferential elimination of repeated DNA sequences from the paternal, Nicotiana tomentosiformis genome donor of a synthetic, allotetraploid tobacco.
The data demonstrate that genetic changes in synthetic tobacco were fast, targeted to the paternal N. tomentosiformis-donated genome, and some of the changes showed concordance with changes that presumably occurred during evolution of natural tobacco.
The origin of tobacco's T genome is traced to a particular lineage within Nicotiana tomentosiformis (Solanaceae).
It is shown that the repetitive sequences of geminiviral origin, GRD53 and GRD3, are present in the genomes of N. tabacum cultivars, a tobacco cell suspension culture TBY-2, and N. tomentosiformis.
Genome evolution in allotetraploid Nicotiana
Overall, only in tobacco is there any evidence that NCI may have influenced genome evolution, and here further data are required to verify chromosome identity.
The molecular cytogenetics of Vigna unguiculata (L.) Walp: the physical organization and characterization of 18s-5.8s-25s rRNA genes, 5s rRNA genes, telomere-like sequences, and a family of centromeric repetitive DNA sequences
Degenerate telomeric repeats gave hybridization signals at the telomeres of most chromosomes and no intercalary sites were detected at metaphase; the sequences appear to have no preferential distribution in interphase nuclei.
Long-term genome diploidization in allopolyploid Nicotiana section Repandae (Solanaceae).
Sequence analysis indicates that 35S and 5S units most closely resemble maternal and paternal progenitors, respectively, in recent Nicotiana allopolyploid section Repandae.
Comparative genomics and repetitive sequence divergence in the species of diploid Nicotiana section Alatae.
This work reconstructs the history of karyotype and tandem repeat evolution in species of diploid Nicotiana section Alatae and suggests that sequence homogenization has replaced HRS60 family repeats at sub-telomeric regions, but that this process may not occur, or occurs more slowly, when the repeats are found at intercalary locations.


Comparison of the mitochondrial genome of Nicotiana tabacum with its progenitor species
Results indicate that the mitochondrial DNA of N. tabacum was inherited from N. sylvestris, and pattern variations indicate that extensive rearrangement of mtDNA has occurred in the evolution of these Nicotiana species.
Genomic in situ hybridization reveals the allopolyploid nature ofMilium montianum (Gramineae)
Genomic in situ hybridization is a potentially powerful tool for studying genome evolution and biosystematics and will often be useful for investigating the origins of wild and cultivated polyploid plant species, especially where conventional methods have failed.
The present publication is concerned with amphiploids between N .
Genome reorganization in Nicotiana asymmetric somatic hybrids analysed by in situ hybridization.
Genomic in situ hybridization to plant chromosomes provides a rapid and reliable means of screening for recombinant genotypes in asymmetric somatic hybrids and contributes to a greater understanding of the events responsible for genomic recovery and restabilization following genetic manipulation in vitro.
Absence of some truncated genes in the amphidiploid Nicotiana tabacum.
Origin of Nicotiana tabacum L. detected by polypeptide composition of Fraction I protein
It is reported that N. tabacum arose from the hybridisation of N. sylvestris and N. tomentosiformis, and the putative progenitor species was N. otophora, a species in the Tomentosae section of Nicotiana.
A BamHI family of highly repeated DNA sequences of Nicotiana tabacum
Results suggest a tandem arrangement of this DNA repeat unit, which comprised about 2% of the nuclear genome of N. tabacum, and computer comparisons with other tandem plant-repeated DNA sequences could not detect any other homologous sequence.
Genomic divergence of allopatric sibling species studied by molecular cytogenetics of their F1hybrids
The ability to discriminate rapidly and reliably between the chromosomes of close relatives with almost identical karyotypes makes GISH invaluable in preliminary studies of phytogeny and confirms the sensitivity of the technique and demonstrates its potential use in evolutionary cytogenetics.
  • S. J. Sheen
  • Biology
    Evolution; international journal of organic evolution
  • 1972
The evolutionary process of N. tabacum may be clarified if synthesis of this species can be achieved through experimental hybridization and amphiploidy, and recently, Cameron (1965) added evidence that N. sylvestris contributed cytoplasm to the hybrid from which N.tabacum was evolved.
Tobacco single-copy DNA is highly homologous to sequences present in the genomes of its diploid progenitors
The results show that significant single-copy DNA sequence divergence has occurred between the diploid N. sylvestris and N. tomentosiformis genomes, but by applying the experimental criteria these single- copy DNAs are indistinguishable from their counterparts in the hybrid N. tabacum nucleus.