Characterization of the NAD+ glycohydrolase associated with the rat liver nuclear envelope.

@article{Fukishima1976CharacterizationOT,
  title={Characterization of the NAD+ glycohydrolase associated with the rat liver nuclear envelope.},
  author={M Fukishima and Hiroto Okayama and Yasuhiro Takahashi and Osamu Hayaishi},
  journal={Journal of biochemistry},
  year={1976},
  volume={80 1},
  pages={167-76}
}
The localization of NAD+ glycohydrolase [EC 3.2.2.5] (NADase) in purified rat liver nuclei has been examined. Subnuclear fractionation revealed that at least 70% of the NADase in nuclei was associated with the nuclear envelope fraction. The nuclear envelope fraction was practically free of microsomal contamination as judged by electron microscopic morphometry and assays of microsomal marker enzymes. Therefore, NADase was found to be an integral component of the nuclear envelope. The… CONTINUE READING