Characterization of the Lipid Binding Properties of Otoferlin Reveals Specific Interactions between PI(4,5)P2 and the C2C and C2F Domains

@inproceedings{Padmanarayana2014CharacterizationOT,
  title={Characterization of the Lipid Binding Properties of
Otoferlin Reveals Specific Interactions between PI(4,5)P2 and the
C2C and C2F Domains},
  author={Murugesh Padmanarayana and Nicole Hams and Lee Colyer Speight and E James Petersson and Ryan A Mehl and Colin P Johnson},
  booktitle={Biochemistry},
  year={2014}
}
Otoferlin is a transmembrane protein consisting of six C2 domains, proposed to act as a calcium sensor for exocytosis. Although otoferlin is believed to bind calcium and lipids, the lipid specificity and identity of the calcium binding domains are controversial. Further, it is currently unclear whether the calcium binding affinity of otoferlin quantitatively matches the maximal intracellular presynaptic calcium concentrations of ∼30-50 μM known to elicit exocytosis. To characterize the calcium… CONTINUE READING
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