Characterization of the Early Lesion of ‘Degenerative’ Valvular Aortic Stenosis: Histological and Immunohistochemical Studies

  title={Characterization of the Early Lesion of ‘Degenerative’ Valvular Aortic Stenosis: Histological and Immunohistochemical Studies},
  author={Catherine M. Otto and Johanna Kuusisto and Dennis D. Reichenbach and Allen M. Gown and Kevin D. O’Brien},
BackgroundNonrheumatic stenosis of trileaflet aortic valves, often termed senile or calcific valvular aortic stenosis, is considered a “degenerative” process, but little is known about the cellular or molecular factors that mediate its development. Methods and ResultsTo characterize the developing aortic valvular lesion, we performed histological and immunohisto-chemical studies on Formalin-fixed and methanol-Carnoy's-fixed parafflin-embedded aortic valve leaflets or on frozen sections obtained… 

Figures and Tables from this paper

Calcification of bicuspid aortic valves
Immunohistochemical studies of trileaflet aortic valves with varying degrees of valve stenosis have established the presence of T lymphocytes and macrophages both in the early stages of this disease process and in valves with severe stenosis removed at surgery.
Osteopontin is expressed in human aortic valvular lesions.
Calcification in aortic stenosis may be, in part, an actively regulated process with the potential for control either through modification of inflammation or synthesis of proteins such as osteopontin, which may modulate calcification in this tissue.
Intraleaflet hemorrhages are a common finding in symptomatic aortic and mitral valves.
Inflammatory Characteristics of Stenotic Aortic Valves: A Comparison between Rheumatic and Nonrheumatic Aortic Stenosis
The similarities regarding the localisation of calcification indicate that the valve anomaly/morphology can influence the pathogenesis of aortic stenosis, and highlights the question of a postinflammatory valvular disease of other causes than rheumatic fever.
Age Depending Characteristics of Aortic Valves Stenosis : Senile versus Atherosclerotic Diseases According to Immunohistochemical Study
  • Medicine, Biology
  • 2016
It was reported that endothelium has different activation in aortic valve stenosis caused by senile versus atherosclerosis and a vascular valve leaflets shoved neoangiogenic profiles directly dependent on active inflammation.
Mechanical and Histological Characterization of Porcine Aortic Valves under Normal and Hypercholesterolemic Conditions
Micropipette aspiration was shown to be capable of measuring the mechanical properties of a single layer in multilayered biomaterial or tissue such as the aortic valve, if the pipette inner diameter was less than the top layer thickness.
Molecular and cellular mechanisms of aortic stenosis.
Cells of primarily extra-valvular origin in degenerative aortic valves and bioprostheses.
EPCs and DCs were detected in a large collective of degenerative aortic valves, more frequently in bioprostheses than in native cusps, and aortoluminal presence of these primarily extra-valvular cells co-localized with inflammatory cells is a novel key feature involved in aortsic valve degeneration.


Minor congenital variations of cusp size in tricuspid aortic valves. Possible link with isolated aortic stenosis.
In this study the width and height of individual aortic valve cusps were measured at necropsy in 200 hearts of patients without clinical signs of aorta stenosis, though the majority of valves obtained from elderly persons showed some ageing changes, and the results show that inequality in cusp size is the rule rather than the exception.
Site of intimal rupture or erosion of thrombosed coronary atherosclerotic plaques is characterized by an inflammatory process irrespective of the dominant plaque morphology.
The underlying atherosclerotic plaque morphology in complicated coronary artery lesions causing acute myocardial infarction is heterogeneous with respect to both plaque architecture and cellular composition, however, the immediate site of plaque rupture or erosion is always marked by an inflammatory process.
Human atherosclerosis. II. Immunocytochemical analysis of the cellular composition of human atherosclerotic lesions.
These studies demonstrate the application of monoclonal antibody technology to the study of the cellular composition of human atherosclerotic plaques with considerable variation from region to region.
Valvular calcification in the elderly: possible pathogenic factors.
Calcification of the mitral ring and of the aortic valve cusps was studied in an unselected autopsy series of 219 patients over 60, dying in a department of geriatric medicine. Possible correlations