Characterization of the DNA-unwinding activity of human RECQ1, a helicase specifically stimulated by human replication protein A.

@article{Cui2003CharacterizationOT,
  title={Characterization of the DNA-unwinding activity of human RECQ1, a helicase specifically stimulated by human replication protein A.},
  author={Sheng Cui and Raffaella Klima and Alex Ochem and Daniele Arosio and A. Falaschi and Alessandro Vindigni},
  journal={The Journal of biological chemistry},
  year={2003},
  volume={278 3},
  pages={1424-32}
}
The RecQ helicases are involved in several aspects of DNA metabolism. Five members of the RecQ family have been found in humans, but only two of them have been carefully characterized, BLM and WRN. In this work, we describe the enzymatic characterization of RECQ1. The helicase has 3' to 5' polarity, cannot start the unwinding from a blunt-ended terminus, and needs a 3'-single-stranded DNA tail longer than 10 nucleotides to open the substrate. However, it was also able to unwind a blunt-ended… CONTINUE READING

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